Research Associate/Senior Research Associate, Stem Cell Biology

About the Job

Colossal is looking for a talented Research Associate in stem cell biology with an interest in the generation, editing and differentiation of stem cells from non-model mammalian organisms. You will work closely with other lab team members in efforts to innovate on protocols of genome editing, reprogramming and lineage differentiation using cutting edge technologies. 

This position is based on-site in Dallas, TX. Relocation assistance is available. 

Duties and Responsibilities: 

• Derive and characterize stable cell lines from primary tissues 
• Reprogram and expand iPSCs from non-model mammalian organisms
• Test novel cell culture conditions and delivery methods to optimize gene editing
• Expand clonal cell lines for cryopreservation, genotyping and QC assays
• Develop cell differentiation assays to perform genotype to phenotype predictions 
• Work with team members to integrate targeting, genotyping and QC workflows 
• Schedule, analyze and execute experiments to deliver on project milestones
• Attend lab meetings, prepares reports and presentations on the progress of projects
• Demonstrate great organizational skills and excellent laboratory record maintenance

Required Skills and Abilities:

• Experience in primary cell line derivation, reprograming, iPSC derivation and culture
• Experience with electroporation and transfection to maximize gene delivery and viability 
• Experience in molecular biology (nucleic and protein extraction, gel electrophoresis) 
• Experience with qPCR, immunostaining, flow cytometry and fluorescent microscopy
• Organized and comfortable multi-tasking to deliver on concurrent projects on time 
• Strong written and oral communication skills, as well as creative problem-solving skills

Preferred Skills and Abilities:

• Experience with high throughput imaging platforms and data analysis
• Experience with CRISPR library construction and QC assay

Education and Experience:

• BA/BS/MA/MS in biological sciences, bioengineering, molecular biology, genetics, or any science related field.