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Protein Expression Scientist
11 days ago
Full time role
In-person · West Point, PA, US
- Perform large-scale (up to 10 L) expression of recombinant proteins and virus-like particles (VLPs) by transient transfection of suspension mammalian cells across multiple expression systems, including shake flask and WAVE bioreactor technology
- Optimize expression conditions for challenging proteins, including multimeric proteins, to improve yield and quality
- Maintain and expand mammalian cell lines, ensuring proper cell density, viability, and sterility
- Operate and maintain cell culture analyzers, including the BioProfile FLEX analyzer, to monitor cell culture health and productivity
- Conduct protein characterization using techniques such as SDS-PAGE, Western blot analysis, and UV-VIS spectroscopy
- Troubleshoot and resolve issues related to protein expression, cell culture, and equipment operation
- Maintain detailed records of experiments, protocols, and results under company policies and regulatory requirements
- Work closely with a lead scientist in the expression group and coordinate with the protein purification and characterization groups in the facility
- Contribute to the development and optimization of new protein expression protocols and techniques
- Participate in team meetings and present findings to colleagues and management
- Ensure compliance with all safety protocols and regulations in the laboratory environment
- Assist in training junior team members on protein expression techniques and equipment usage
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